Protein association of the neurotoxin and non-protein amino acid BMAA (β-N-methylamino-l-alanine) in the liver and brain following neonatal administration in rats

Karlsson O, L. Jiang, Andersson M, L. Ilag, Brittebo EB
2014 | 226(1) (1-5)

The environmental neurotoxin β-N-methylamino-l-alanine (BMAA) is not an amino acid that is normally found in proteins. Our previous autoradiographic study of (3)H-labeled BMAA in adult mice unexpectedly revealed a tissue distribution similar to that of protein amino acids. The aim of this study was to characterize the distribution of free and protein-bound BMAA in neonatal rat tissues following a short exposure using autoradiographic imaging and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The autoradiographic imaging of (14)C-L-BMAA demonstrated a distinct uptake of radioactivity that was retained following acid extraction in tissues with a high rate of cell turnover and/or protein synthesis. The UHPLC-MS/MS analysis conclusively demonstrated a dose-dependent increase of protein-associated BMAA in neonatal rat tissues. The level of protein-associated BMAA in the liver was more than 10 times higher than that in brain regions not fully protected by the blood-brain barrier which may be due to the higher rate of protein synthesis in the liver. In conclusion, this study demonstrated that BMAA was associated with rat proteins suggesting that BMAA may be misincorporated into proteins. However, protein-associated BMAA seemed to be cleared over time, as none of the samples from adult rats had any detectable free or protein-associated BMAA.

Repeated measurements of per- and polyfluoroalkyl substances (PFASs) from 1979 to 2007 in males from Northern Norway: Assessing time trends, compound correlations and relations to age/birth cohort.

Haugdahl Nøst, T.; Vestergren, R.; Berg, V.; Nieboer, E.; Odland, J.Ø.; Sandanger, T.M.
2014 | Environ Int | 67C (43-53)

Are we done with the research on sources of C4–C14 perfluoroalkyl carboxylic acids (PFCAs)? Lessons learned from recent identification of overlooked sources.

Wang, Z.; Cousins, I.T.; Scheringer, M.; Buck, R.C.; Hungerbühler, K.
2014 | SU

6th International Workshop on Per- and Polyfluorinated Alkyl Substances – PFASs | September 19, 2019 | Idstein, Germany

Equilibrium Sampling to Determine the Thermodynamic Potential for Bioaccumulation of Persistent Organic Pollutants from Sediment

Jahnke, A.; MacLeod, M.; Wickström, H.; Mayer, P.
2014 | Environ. Sci. Technol. | 48

Equilibrium partitioning (EqP) theory is currently the most widely used approach for linking sediment pollution by persistent hydrophobic organic chemicals to bioaccumulation. Most applications of the EqP approach assume (I) a generic relationship between organic carbon-normalized chemical concentrations in sediments and lipid-normalized concentrations in biota and (II) that bioaccumulation does not induce levels exceeding those expected from equilibrium partitioning. Here, we demonstrate that assumption I can be obviated by equilibrating a silicone sampler with chemicals in sediment, measuring chemical concentrations in the silicone, and applying lipid/silicone partition ratios to yield concentrations in lipid at thermodynamic equilibrium with the sediment (CLip⇌Sed). Furthermore, we evaluated the validity of assumption II by comparing CLip⇌Sed of selected persistent, bioaccumulative and toxic pollutants (polychlorinated biphenyls (PCBs) and hexachlorobenzene (HCB)) to lipid-normalized concentrations for a range of biota from a Swedish background lake. PCBs in duck mussels, roach, eel, pikeperch, perch and pike were mostly below the equilibrium partitioning level relative to the sediment, i.e., lipid-normalized concentrations were ≤CLip⇌Sed, whereas HCB was near equilibrium between biota and sediment. Equilibrium sampling allows straightforward, sensitive and precise measurement of CLip⇌Sed. We propose CLip⇌Sed as a metric of the thermodynamic potential for bioaccumulation of persistent organic chemicals from sediment useful to prioritize management actions to remediate contaminated sites.

Assessing Metabolomic Response in Multiple Aquatic Species Exposed to Effluents, Water, and Sediments from Hamilton Harbour, Ontario

Benskin JP, Watson-Leung T, Kleywegt S, Chandramouli B, Tketchva V, Helm P.
2014 | Society of Environmental Toxicology and Chemistry (SETAC)

SETAC NA Conference | September 19, 2019 | Vancouver

Future Nutrient Load Scenarios for the Baltic Sea Due to Climate and Lifestyle Changes

Eriksson Hägg, H; Lyon, S.W.; Wällstedt, T.; Mörth, C.-M.; Claremar, B.; Humborg, C.
2014 | Ambio | 43 (337-351)

Effect of Ammonia on the Volatility of Organic Diacids

Paciga, Andrea L.; Riipinen, Ilona; Pandis, Spyros N.
2014 | Environ. Sci. Technol. | 48 (23) (13769-13775)

The effect of ammonia on the partitioning of two dicarboxylic acids, oxalic (C2) and adipic (C6) is determined. Measurements by a tandem differential mobility analysis system and a thermodenuder (TD-TDMA) system are used to estimate the saturation vapor pressure and enthalpy of vaporization of ammonium oxalate and adipate. Ammonia dramatically lowered the vapor pressure of oxalic acid, by several orders of magnitude, with an estimated vapor pressure of 1.7 +/- 0.8 x 10(-6) Pa at 298 K. The vapor pressure of ammonium adipate was 2.5 +/- 0.8 x 10(-5) Pa at 298 K, similar to that of adipic acid. These results suggest that the dominance of oxalate in diacid concentrations measured in ambient aerosol could be attributed to the salt formation with ammonia.

Preparation of monolithic molecularly imprinted polymer sol–gel packed tips for high-throughput bioanalysis: Extraction and quantification of l-tyrosine in human plasma and urine samples utilizing liquid chromatography and tandem mass spectrometry

M. Mahdi Moein, A. El Beqqali, Abbi Abdel-Rehim, Amin Jeppsson-Dadoun, M. Abdel-Rehim
2014 | J. Chromatogr. B | 967 (168-173)

In situ monolithic molecularly imprinted polymer sol–gel packed tips (MMSTs) were prepared and evaluated for the extraction of lung cancer biomarker l-tyrosine (Tyr) from human plasma and urine samples. Several extraction parameters such as the conditioning, washing and elution solutions, pH and time were investigated. The enrichment factor (EF) and extraction recovery (ER) were studied. MMST showed good selectivity and a high extraction recovery, and MMST as a sorbent showed good stability and repeatability. The method validation showed good regression correlation coefficients for plasma and urine samples (R2 ≥ 0.996) within the concentration range of 5–1000 and 1–1000 nmol L−1 in plasma and urine samples, respectively. The lower limits of quantification (LLOQ) in the plasma and urine samples were 5 and 1 nmol L−1, respectively. The between-batch precision for Tyr in plasma ranged from 1.0 to 6.0%, and in urine it was from 1.0 to 7.0%. The results show that the developed method has more facility, stability, durability and repeatability compared with previous similar methods. To the best of our knowledge, this is the first study aimed at the selective separation of Tyr as a lung cancer biomarker by MMSTs from biological matrixes and detection by LC/MS/MS.

Temporal trends of perfluoroalkane sulfonic acids and their sulfonamide-based precursors in herring from the Swedish west coast 1991-2011 including isomer-specific considerations.

Ullah, S.; Huber, S.; Bignert, A.; Berger, U.
2014 | Environ Int | 65C (63-72)

A method was developed for simultaneous analysis of perfluoroalkane sulfonic acids (PFSAs) and their sulfonamide-based precursors (perfluoroalkane sulfonamidoacetic acids (FASAAs), sulfonamides (FASAs), and sulfonamidoethanols (FASEs)) in fish muscle. Extraction was performed with acetonitrile followed by a clean-up and fractionation step and instrumental analysis by UPLC/MS/MS and GC/MS. Time trends of PFSAs and their precursors in herring muscle samples originating from the Kattegat at the west coast of Sweden were investigated covering the years 1991-2011. The following analytes were detected, all with decreasing or unchanged trends between 1991 and 2011: Perfluorobutane sulfonic acid (PFBS, below the method detection limit (

Integrated approach for the identification, prioritisation and abatement of emerging pollutants – a SOLUTIONS – oriented approach

Brack, W.; Barcelo, D.; Altenburger, R.; Bunke, D.; Cousins, I.T.; Dimitrov, S.; Engelen, G.; Faust, M.; Gawlik, M.; Hollender, J.; Kortenkamp, A.; Lopez Herraez, D.; Munthe, M.; Posthuma, L.; Slobodnik, J.; Tollefsen, K.-N.; van den Brink, P.; van Gils, J.; van Wezel, A-M.
2014 | SU

SETAC Europe 24th Annual Conference | September 19, 2019 | Basel, Switzerland

Hygroscopicity, CCN and volatility properties of submicron atmospheric aerosol in a boreal forest environment during the summer of 2010

Hong, J; Hakkinen, SAK; Paramonov, M; Aijala, M; Hakala, J; Nieminen, T; Mikkila, J; Prisle, NL; Kulmala, M; Riipinen, I; Bilde, M; Kerminen, VM; Petaja, T
2014 | Atmos. Chem. Phys. | 14 (9) (4733-4748)

A Volatility-Hygroscopicity Tandem Differential Mobility Analyzer (VH-TDMA) was applied to study the hygroscopicity and volatility properties of submicron atmospheric aerosol particles in a boreal forest environment in Hyytiala, Finland during the summer of 2010. Aitken and accumulation mode internally mixed particles (50 nm, 75 nm and 110 nm in diameter) were investigated. Hygroscopicity was found to increase with particle size. The relative mass fraction of organics and SO42- is probably the major contributor to the fluctuation of the hygroscopicity for all particle sizes. The Cloud Condensation Nuclei Counter (CCNC)-derived hygroscopicity parameter kappa was observed to be slightly higher than kappa calculated from VH-TDMA data under sub-saturated conditions, potential reasons for this behavior are discussed shortly. Also, the size-resolved volatility properties of particles were investigated. Upon heating, more small particles evaporated compared to large particles. There was a significant amount of aerosol volume (non-volatile material) left, even at heating temperatures of 280 degrees C. Using size resolved volatility-hygroscopicity analysis, we concluded that there was always hygroscopic material remaining in the particles at different heating temperatures, even at 280 degrees C. This indicates that the observed non-volatile aerosol material did not consist solely of black carbon.

Provningsjämförelse / Proficiency Test 2014-4, Närsalter / Nutrients

2014 | Report, ITM, SU | ISBN: 1103-341 | Report No: 227

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