Preparation and characterization of subcellular fractions from the liver of the northern pike, Esox lucius

Balk, L.; Meijer, J.; Bergstrand, A.; Åström, A.; Morgenstern, R.; Seidegård, J.; DePierre, J.W.
1982 | Biochem. Pharmacol. | 31 (1491-1500)

The present study was designed to prepare and characterize subcellular fractions from the liver of the Northern pike (Esox lucius), with special emphasis on the preparation of microsomal fractions suitable for studying xenobiotic metabolism. The purity of the different fractions obtained by differential centrifugation, as well as the recovery of different organelles, was determined using both enzyme markers and morphological examination with the electron microscope. Attempts were also made to increase the recovery of fragments of the endoplasmic reticulum in the microsomal fraction. Finally, the subcellular distribution of several drug-metabolizing enzymes (cytochrome P-450, benzpyrene monooxygenase, epoxide hydrolase and glutathione transferases) were determined. With the exception of the subcellular distribution of epoxide hydrolase, the results obtained here resemble closely those reported for rat liver and the microsomal fraction prepared is highly suitable for further studies of drug metabolism in pike liver.

Metabolism of 2,4′,5-trichlorobiphenyl: Enrichment of hydroxylated and methyl sulphone metabolites in the uterine luminal fluid of pregnant mice

Brandt I; Darnerud PO; Bergman Å; Larsson Y;
1982 | Chem. Biol. Interact. | 40

Shpol’skii Effect in the Analysis of Sulfur-Containing Heterocyclic Aromatic Compounds

A. Colmsjö, Zebühr Y , C. Östman
1982 | 54 (1673-1677)

Separation of the different classes of conjugates formed by metabolism of benzol[a]pyrene in the northern pike (Esox lucius)

Balk, L.; Knall, A.; DePierre, J.W.
1982 | Acta Chem. Scand., B, Org. Chem. Biochem. | 36 (403-405)

Metabolism of 2,4′,5-trichlorobiphenyl by the mercapturic acid pathway

Bakke JE; Bergman Å; Larsen GL;
1982 | Science | 217

Metabolism of 2,4′,5-trichlorobiphenyl: Tissue concentrations of methylsulphonyl-2,4′,5-trichlorobiphenyl in germfree and conventional mice

Brandt I; Klasson Wehler E; Rafter J; Bergman Å;
1982 | Toxicol. Lett. | 12

Distribution of 3H-benzo(a)pyrene to different body compartments in the Northern pike, Esox lucius

Balk, L.; Meijer, J.; DePierre, J.W.
1981 | Symposium on Fish Physiology

Third Symposium on Fish Physiology | August 17, 2019 | Bangor, Wales, UK

Synthesis of polychlorinated [14C]alkanes (PCA) of high specific activity

Bergman Å; Leonardsson I; Wachtmeister CA;
1981 | Chemosphere | 10

Induction of drug-metabolizing enzymes : a status report

DePierre, J.W.; Seidegård, J.; Morgenstern, R.; Balk, L.; Meijer, J.; Åström, A.
1981 | SU (585-610) | ISBN: 0-201-04576-1

THE CHEMICAL-COMPOSITION OF ARCTIC HAZE AT NY-ALESUND, SPITSBERGEN

HEINTZENBERG, J; HANSSON, HC; LANNEFORS, H
1981 | Tellus | 33 (2) (162-171)

Storskalig odling av encelliga marina alger.

B. Eklund, E. Sahlsten
1981

NON-URBAN AEROSOL COMPOSITION NEAR BEIJING, CHINA

WINCHESTER, JW; WANG, MX; REN, LX; LU, WX; HANSSON, HC; LANNEFORS, H; DARZI, M; LESLIE, ACD
1981 | NUCLEAR INSTRUMENTS & METHODS | 181 (1-3) (391-398)

Contact information

Visiting addresses:

Geovetenskapens Hus,
Svante Arrhenius väg 8, Stockholm

Arrheniuslaboratoriet, Svante Arrhenius väg 16, Stockholm (Unit for Analytical and Toxicological Chemistry)

Mailing address:
Department of Environmental Science and Analytical Chemistry (ACES)
Stockholm University
106 91 Stockholm

Press enquiries should be directed to:

Stella Papadopoulou
Science Communicator
Phone +46 (0)8 674 70 11
stella.papadopoulou@aces.su.se