Identifying precursors and aqueous organic aerosol formation pathways during the SOAS campaign

Sareen, Neha; Carlton, Annmarie G.; Surratt, Jason D.; Gold, Avram; Lee, Ben; Lopez-Hilfiker, Felipe D.; Mohr, Claudia; Thornton, Joel A.; Zhang, Zhenfa; Lim, Yong B.; Turpin, Barbara J.
2016 | Atmos. Chem. Phys. | 16 (22) (14409-14420)

Aqueous multiphase chemistry in the atmosphere can lead to rapid transformation of organic compounds, forming highly oxidized, low-volatility organic aerosol and, in some cases, light-absorbing (brown) carbon. Because liquid water is globally abundant, this chemistry could substantially impact climate, air quality, and health. Gas-phase precursors released from biogenic and anthropogenic sources are oxidized and fragmented, forming water-soluble gases that can undergo reactions in the aqueous phase (in clouds, fogs, and wet aerosols), leading to the formation of secondary organic aerosol (SOAAQ/. Recent studies have highlighted the role of certain precursors like glyoxal, methylglyoxal, glycolaldehyde, acetic acid, acetone, and epoxides in the formation of SOAAQ. The goal of this work is to identify additional precursors and products that may be atmospherically important. In this study, ambient mixtures of water-soluble gases were scrubbed from the atmosphere into water at Brent, Alabama, during the 2013 Southern Oxidant and Aerosol Study (SOAS). Hydroxyl (OH center dot) radical oxidation experiments were conducted with the aqueous mixtures collected from SOAS to better understand the formation of SOA through gas-phase followed by aqueous-phase chemistry. To-tal aqueous-phase organic carbon concentrations for these mixtures ranged from 92 to 179 mu M-C, relevant for cloud and fog waters. Aqueous OH-reactive compounds were primarily observed as odd ions in the positive ion mode by electrospray ionization mass spectrometry (ESI-MS). Ultra high-resolution Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) spectra and tandem MS (MS-MS) fragmentation of these ions were consistent with the presence of carbonyls and tetrols. Products were observed in the negative ion mode and included pyruvate and oxalate, which were confirmed by ion chromatography. Pyruvate and oxalate have been found in the particle phase in many locations (as salts and complexes). Thus, formation of pyruvate/oxalate suggests the potential for aqueous processing of these ambient mixtures to form SOA(AQ).

Evaluation of European air quality modelled by CAMx including the volatility basis set scheme

Ciarelli, Giancarlo; Aksoyoglu, Sebnem; Crippa, Monica; Jimenez, Jose-Luis; Nemitz, Eriko; Sellegri, Karine; Aijala, Mikko; Carbone, Samara; Mohr, Claudia; O'Dowd, Colin; Poulain, Laurent; Baltensperger, Urs; Prevot, Andre S. H.
2016 | Atmos. Chem. Phys. | 16 (16) (10313-10332)

Four periods of EMEP (European Monitoring and Evaluation Programme) intensive measurement campaigns (June 2006, January 2007, September-October 2008 and February-March 2009) were modelled using the regional air quality model CAMx with VBS (volatility basis set) approach for the first time in Europe within the framework of the EURODELTA-III model intercomparison exercise. More detailed analysis and sensitivity tests were performed for the period of February-March 2009 and June 2006 to investigate the uncertainties in emissions as well as to improve the modelling of organic aerosol (OA). Model performance for selected gas phase species and PM2.5 was evaluated using the European air quality database AirBase. Sulfur dioxide (SO2) and ozone (O-3) were found to be overestimated for all the four periods, with O-3 having the largest mean bias during June 2006 and January-February 2007 periods (8.9 pbb and 12.3 ppb mean biases respectively). In contrast, nitrogen dioxide (NO2) and carbon monoxide (CO) were found to be underestimated for all the four periods. CAMx reproduced both total concentrations and monthly variations of PM2.5 for all the four periods with average biases ranging from -2.1 to 1.0 mu g m(-3). Comparisons with AMS (aerosol mass spectrometer) measurements at different sites in Europe during February-March 2009 showed that in general the model overpredicts the inorganic aerosol fraction and underpredicts the organic one, such that the good agreement for PM2.5 is partly due to compensation of errors. The effect of the choice of VBS scheme on OA was investigated as well. Two sensitivity tests with volatility distributions based on previous chamber and ambient measurements data were performed. For February-March 2009 the chamber case reduced the total OA concentrations by about 42% on average. In contrast, a test based on ambient measurement data increased OA concentrations by about 42% for the same period bringing model and observations into better agreement. Comparison with the AMS data at the rural Swiss site Payerne in June 2006 shows no significant improvement in modelled OA concentration. Further sensitivity tests with increased biogenic and anthropogenic emissions suggest that OA in Payerne was affected by changes in emissions from residential heating during the February-March 2009 whereas it was more sensitive to biogenic precursors in June 2006.

Carbon isotope composition of carbohydrates and polyols in leaf and phloem sap of Phaseolus vulgaris L. influences predictions of plant water use efficiency

Smith M.; Wild B.; Simonin K.; Richter A.; Merchant A.
2016 | Plant Cell Physiol. | 57 (1756-1766)

Enhanced availability of mercury bound to dissolved organic matter for methylation in marine sediments

Mazrui, NM; Jonsson, S; Thota, S; Zhao, J; Mason, RP
2016 | Geochim. Cosmochim. Acta | 194 (153-162)
bioavailability , complexation , dissolution , dom , estuarine sediment , fluorescence , marine , mercury , methylation , methylmercury production , rates , sediment , sulfate-reducing bacteria , sulfide nanoparticles
The forms of inorganic mercury (Hg-II) taken up and methylated by bacteria in sediments still remain largely unknown. From pure cultures studies, it has been suggested that dissolved organic matter (DOM) may facilitate the uptake either by acting as a shuttle molecule, transporting the Hg-II atom to divalent metal transporters, or by binding Hg-II and then being transported into the cell as a carbon source. Enhanced availability of Hg complexed to DOM has however not yet been demonstrated in natural systems. Here, we show that Hg-II complexed with DOM of marine origin was up to 2.7 times more available for methylation in sediments than Hg-II added as a dissolved inorganic complex (Hg-II (aq)). We argue that the DOM used to complex Hg-II directly facilitated the bacterial uptake of Hg-II whereas the inorganic dissolved Hg-II complex adsorbed to the sediment matrix before forming bioavailable dissolved Hg-II complexes. We further demonstrate that differences in net methylation in sediments with high and low organic carbon content may be explained by differences in the availability of carbon to stimulate the activity of Hg methylating bacteria rather than, as previously proposed, be due to differences in Hg-II binding capacities between sediments. (C) 2016 Elsevier Ltd. All rights reserved.

Urinary bisphenol A is associated with dysregulation of HPA-axis function in pregnant women: Findings from the APrON cohort study

Giesbrecht, GF; Liu, JY; Ejaredar, M; Dewey, D; Letourneau, N; Campbell, T; Martin, JW
2016 | Environ. Res. | 151 (689-697)
behavior , birth outcomes , bisphenol a , cortisol , cortisol awakening response , depression scale , hpa-axis function , mice , perinatal exposure , phthalate , pregnancy , prenatal exposure , rats , stress physiology

Background: Bisphenol A (BPA) is associated with dysregulation of hypothalamic-pituitary-adrenal (HPA) axis activity in rodents, but evidence in humans is lacking. Objective: To determine whether BPA exposure during pregnancy is associated with dysregulation of the HPA-axis, we examined the association between urinary BPA concentrations and diurnal salivary cortisol in pregnant women. Secondary analyses investigated whether the association between BPA and cortisol was dependent on fetal sex. Methods: Diurnal salivary cortisol and urinary BPA were collected during pregnancy from 174 women in a longitudinal cohort study, the Alberta Pregnancy Outcomes and Nutrition (APrON) study. Associations between BPA and daytime cortisol and the cortisol awakening response (CAR) were estimated using mixed models after adjusting for covariates. Results: Higher concentrations of total BPA uncorrected for urinary creatinine were associated with dysregulation of the daytime cortisol pattern, including reduced cortisol at waking, beta = -.055, 95% CI (-.100, -.010) and a flatter daytime pattern, beta = .014, 95% CI (.006, .022) and beta = -.0007 95% CI (-.001, -.0002) for the linear and quadratic slopes, respectively. Effect sizes in creatinine corrected BPA models were slightly smaller. None of the interactions between fetal sex and BPA were significant (all 95% CI's include zero). Conclusions: These findings provide the first human evidence suggesting that BPA exposure is associated with dysregulation of HPA-axis function during pregnancy. (C) 2016 Elsevier Inc. All rights reserved.

Ubiquity of organic nitrates from nighttime chemistry in the European submicron aerosol

Kiendler-Scharr, A.; Mensah, A. A.; Friese, E.; Topping, D.; Nemitz, E.; Prevot, A. S. H.; Aijala, M.; Allan, J.; Canonaco, F.; Canagaratna, M.; Carbone, S.; Crippa, M.; Dall Osto, M.; Day, D. A.; De Carlo, P.; Di Marco, C. F.; Elbern, H.; Eriksson, A.; Freney, E.; Hao, L.; Herrmann, H.; Hildebrandt, L.; Hillamo, R.; Jimenez, J. L.; Laaksonen, A.; McFiggans, G.; Mohr, C.; O'Dowd, C.; Otjes, R.; Ovadnevaite, J.; Pandis, S. N.; Poulain, L.; Schlag, P.; Sellegri, K.; Swietlicki, E.; Tiitta, P.; Vermeulen, A.; Wahner, A.; Worsnop, D.; Wu, H. -C.
2016 | Geophys Res Lett | 43 (14) (7735-7744)

In the atmosphere nighttime removal of volatile organic compounds is initiated to a large extent by reaction with the nitrate radical (NO3) forming organic nitrates which partition between gas and particulate phase. Here we show based on particle phase measurements performed at a suburban site in the Netherlands that organic nitrates contribute substantially to particulate nitrate and organic mass. Comparisons with a chemistry transport model indicate that most of the measured particulate organic nitrates are formed by NO3 oxidation. Using aerosol composition data from three intensive observation periods at numerous measurement sites across Europe, we conclude that organic nitrates are a considerable fraction of fine particulate matter (PM1) at the continental scale. Organic nitrates represent 34% to 44% of measured submicron aerosol nitrate and are found at all urban and rural sites, implying a substantial potential of PM reduction by NOx emission control.

Plant-derived compounds stimulate the decomposition of organic matter in arctic permafrost soils

Wild B.; Gentsch N.; Čapek P.; Diáková K.; Alves R.J.E.; Bárta J.; Gittel A.; Hugelius G.; Knoltsch A.; Kuhry P.; Lashchinskiy N.; Mikutta R.; Palmtag J.; Schleper C.; Schnecker J.; Shibistova O.; Takriti M.; Torsvik V.L.; Urich T.; Watzka M.; Šantrůčková H.; Guggenberger G.; Richter A.
2016 | Sci Rep | 6

Effects of Nutrient Loading and Mercury Chemical Speciation on the Formation and Degradation of Methylmercury in Estuarine Sediment

Van, LN; Jonsson, S; Skyllberg, U; Nilsson, MB; Andersson, A; Lundberg, E; Bjorn, E
2016 | Environ. Sci. Technol. | 50 (13) (6983-6990)
bioavailability , desulfobulbus-propionicus 1pr3 , dissolved organic matter , fresh-water sediments , icp-ms , iron-reducing bacteria , marine-sediments , methylation rates , pure cultures , sulfate
Net formation of methylmercury (MeHg) in sediments is known to be affected by the availability of inorganic divalent mercury (Hg-II) and by the activities of Hg-II methylating and MeHg demethylating bacteria. Enhanced autochthonous organic matter deposition to the benthic zone, following increased loading of nutrients to the pelagic zone, has been suggested to increase the activity of Hg-II methylating bacteria and thus the rate of net methylation. However, the impact of increased nutrient loading on the biogeochemistry Of mercury (Hg) is challenging to predict as different geochemical pools of Hg may respond differently to enhanced bacterial activities. Here, we investigate the combined effects of nutrient (N and P) supply to the pelagic zone and the chemical speciation of Hg-II and of MeHg on MeHg formation and degradation in a brackish sediment-water mesocosm model ecosystem. By use of Hg isotope tracers added in situ to the mesocosms or ex situ in incubation experiments, we show that the MeHg formation rate increased with nutrient loading only for Hg-II tracers with a high availability for methylation. Tracers with low availability did not respond significantly to nutrient loading. Thus, both microbial activity (stimulated indirectly through plankton biomass production by nutrient loading) and Hg-II chemical speciation were found to control the MeHg formation rate in marine sediments.

Effect of Lipid Partitioning on Predictions of Acute Toxicity of Oil Sands Process Affected Water to Embryos of Fathead Minnow (Pimephales promelas)

Morandi, GD; Zhang, K; Wiseman, SB; Pereira, AD; Martin, JW; Giesy, JP
2016 | Environ. Sci. Technol. | 50 (16) (8858-8866)
aquatic bioassays , bioconcentration qsars , body residues , dissolved organic-compounds , mass spectrometry , naphthenic acids , narcotic organics , ozonation , residue-based interpretation , solid-phase microextraction

Dissolved organic compounds in oil sands process affected water (OSPW) are known to be responsible for most of its toxicity to aquatic organisms, but the complexity of this mixture prevents use of traditional bottom-up approaches for predicting toxicities of mixtures. Therefore, a top-down approach to predict toxicity of the dissolved organic fraction of OSPW was developed and tested. Accurate masses (i.e., m/z) determined by ultrahigh resolution mass spectrometry in negative and positive ionization modes were used to assign empirical chemical formulas to each chemical species in the mixture. For each chemical species, a predictive measure of lipid accumulation was estimated by stir-bar sorptive extraction (SBSE) to poly, (dimethyl)siloxane, or by partitioning to solid-supported lipid membranes (SSLM). A narcosis mode of action was assumed" and the target-lipid model was used-to estimate potencies of mixtures by assuming strict additivity. A model developed using a combination of the SBSE and SSLM lipid partitioning estimates, whereby the accumulation of chemicals to neutral and 'polar lipids was explicitly considered, was best for predicting empirical values of LCSO in 96-h acute toxicity tests with embryos of fathead minnow (Pimephales promelas). Model predictions were within 4-fold of observed toxicity for 75% of OSPW samples, and within 8.5-fold for all samples tested, which is comparable to the range of interlaboratory variability for in vivo toxicity testing.

Molecular Composition and Volatility of Organic Aerosol in the Southeastern US: Implications for IEPDX Derived SOA

Lopez-Hilfiker, F. D.; Mohr, C.; D'Ambro, E. L.; Lutz, A.; Riedel, T. P.; Gaston, C. J.; Iyer, S.; Zhang, Z.; Gold, A.; Surratt, J. D.; Lee, B. H.; Kurten, T.; Hu, W. W.; Jimenez, J.; Hallquist, M.; Thornton, J. A.
2016 | Environ. Sci. Technol. | 50 (5) (2200-2209)

We present measurements as part of the Southern Oxidant and Aerosol Study (SOAS) during which atmospheric aerosol particles were comprehensively characterized. We present results utilizing a Filter Inlet for Gases and AEROsol coupled to a chemical ionization mass spectrometer (CIMS). We focus on the volatility and composition of isoprene derived organic aerosol tracers and of the bulk organic aerosol. By utilizing the online volatility and molecular composition information provided by the FIGAERO-CIMS, we show that the vast majority of commonly reported molecular tracers of isoprene epoxydiol (IEPDX) derived secondary organic aerosol (SOA) is derived from thermal decomposition of accretion products or other low volatility organics having effective saturation vapor concentrations <10(-3) mu g m(-3). In addition, while accounting for up to 30% of total submicrometer organic aerosol mass, the IEPDX-derived SOA has a higher volatility than the remaining bulk. That IEPDX-SOA, and more generally bulk organic aerosol in the Southeastern U.S. is comprised of effectively nonvolatile material has important implications for modeling SOA derived from isoprene, and for mechanistic interpretations of molecular tracer measurements. Our results show that partitioning theory performs well for 2-methyltetrols, once accretion product decomposition is taken into account. No significant partitioning delays due to aerosol phase or viscosity are observed, and no partitioning to particle-phase water or other unexplained mechanisms are needed to explain our results.

Dimethylmercury Formation Mediated by Inorganic and Organic Reduced Sulfur Surfaces

Jonsson, S; Mazrui, NM; Mason, RP
2016 | Sci Rep | 6
atlantic-ocean , cadmium , disordered mackinawite , equatorial pacific , iron-sulfide , isotopes , mackinawite fes , mercury , methylation , water column
Underlying formation pathways of dimethylmercury ((CH3)(2)Hg) in the ocean are unknown. Early work proposed reactions of inorganic Hg (Hg-II) with methyl cobalamin or of dissolved monomethylmercury (CH3Hg) with hydrogen sulfide as possible bacterial mediated or abiotic pathways. A significant fraction (up to 90%) of CH3Hg in natural waters is however adsorbed to reduced sulfur groups on mineral or organic surfaces. We show that binding of CH3Hg to such reactive sites facilitates the formation of (CH3)(2)Hg by degradation of the adsorbed CH3Hg. We demonstrate that the reaction can be mediated by different sulfide minerals, as well as by dithiols suggesting that e.g. reduced sulfur groups on mineral particles or on protein surfaces could mediate the reaction. The observed fraction of CH3Hg methylated on sulfide mineral surfaces exceeded previously observed methylation rates of CH3Hg to (CH3)(2)Hg in seawaters and we suggest the pathway demonstrated here could account for much of the (CH3)(2)Hg found in the ocean.

Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts – Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring

Di Paolo, C; Ottermanns, R; Keiter, S; Ait-Aissa, S; Bluhm, K; Brack, W; Breitholtz, M; Buchinger, S; Carere, M; Chalon, C; Cousin, X; Dulio, V; Escher, BI; Hamers, T; Hilscherova, K; Jarque, S; Jonas, A; Maillot-Marechal, E; Marneffe, Y; Nguyen, MT; Pandard, P; Schifferli, A; Schulze, T; Seidensticker, S; Seiler, TB; Tang, J; van der Oost, R; Vermeirssen, E; Zounkova, R; Zwart, N; Hollert, H
2016 | Water Res. | 104 (473-484)
Bioassays are particularly useful tools to link the chemical and ecological assessments in water quality monitoring. Different methods cover a broad range of toxicity mechanisms in diverse organisms, and account for risks posed by non-target compounds and mixtures. Many tests are already applied in chemical and waste assessments, and stakeholders from the science-police interface have recommended their integration in regulatory water quality monitoring. Still, there is a need to address bioassay suitability to evaluate water samples containing emerging pollutants, which are a current priority in water quality monitoring. The presented interlaboratory study (ILS) verified whether a battery of miniaturized bioassays, conducted in 11 different laboratories following their own protocols, would produce comparable results when applied to evaluate blinded samples consisting of a pristine water extract spiked with four emerging pollutants as single chemicals or mixtures, i.e. triclosan, acridine, 17 alpha-ethinylestradiol (EE2) and 3-nitrobenzanthrone (3-NBA). Assays evaluated effects on aquatic organisms from three different trophic levels (algae, daphnids, zebrafish embryos) and mechanism-specific effects using in vitro estrogenicity (ER-Luc, YES) and mutagenicity (Ames fluctuation) assays. The test battery presented complementary sensitivity and specificity to evaluate the different blinded water extract spikes. Aquatic organisms differed in terms of sensitivity to triclosan (algae > daphnids > fish) and acridine (fish > daphnids > algae) spikes, confirming the complementary role of the three taxa for water quality assessment. Estrogenicity and mutagenicity assays identified with high precision the respective mechanism-specific effects of spikes even when non-specific toxicity occurred in mixture. For estrogenicity, although differences were observed between assays and models, EE2 spike relative induction EC50 values were comparable to the literature, and E2/EE2 equivalency factors reliably reflected the sample content. In the Ames, strong revertant induction occurred following 3-NBA spike incubation with the TA98 strain, which was of lower magnitude after metabolic transformation and when compared to TA100. Differences in experimental protocols, model organisms, and data analysis can be sources of variation, indicating that respective harmonized standard procedures should be followed when implementing bioassays in water monitoring. Together with other ongoing activities for the validation of a basic bioassay battery, the present study is an important step towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring. (C) 2016 Elsevier Ltd. All rights reserved.

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