Polynuclear Aromatic Compounds in the Ambient Air of Stockholm

A. Colmsjö, Söderström H, Wädding A, Zebuhr Y , C. Östman
1986 | 15(2) (169-182)

The abundance of 15 polynuclear aromatic compounds (PAC) in the ambient air in Stockholm have been monitored over a period of four years. Correlation was made between the concentrations of PAC and other simultaneously measured parameters.

Thiophenepropyltrichlorosilane as Chemical Modifier on Silicagel for Reversed Phase Chromatography

Ericsson M , A. Colmsjö
1986 | 21 (451-453)

Modification of silica gel with thiophene-propyl-trichlorosilane is described as well as the synthesis of the silane. The chromatographic performance and selectivity are demonstrated with various polyaromatic compounds. Thiophene-modified silica gel was found to exhibit a selectivity comparable with both ODS-and amino-phases.

Fingerprint Identification of Dibenzopyrenes and Dibenzofluoranthenes

A. Colmsjö, Lindström E, Zebühr Y , C. Östman
1985 | 9 (187-196)

Preparation and characterization of subcellular fractions from the head kidney of the Northern pike (esox lucius), with particular emphasis of xenobiotic-metabolizing enzymes

Balk, L.; Månér, S.; Bergstrand, A.; Birberg, W.; Pilotti, Å; DePierre, J.W.
1985 | Biochem. Pharmacol. | 34 (789-82)

The present study was designed to prepare and characterize subcellular fractions from the head kidney of the Northern pike (Esox lucius), with special emphasis on the preparation of a microsomal fraction suitable for studying xenobiotic metabolism. The purity of the different fractions obtained by differential centrifugation as well as the recovery of different cell components was determined using both enzyme markers and morphological criteria. Finally, the subcellular distributions of several drug-metabolizing enzymes (NADPH-cytochrome c reductase, NADH-ferricyanide reductase, glutathione transferase, epoxide hydrolase) were determined. With the exception of NADPH-cytochrome c reductase, the subcellular distributions obtained here for drug-metabolizing and marker enzymes closely resembled those reported for rat liver. NADPH-cytochrome c reductase was apparently partially solubilized here from microsomal vesicles by an endogenous protease, which reduced its usefulness as a marker enzyme and raises questions concerning the measurement of activities catalyzed by the cytochrome P-450 system in these subfractions. In other respects the microsomal fraction prepared here from the pike head kidney seems well-suited for studies of drug metabolism.

Identification of Chlorinated Polyaromatic Compounds by Shpol’skii Spectroscopy

A. Colmsjö, Zebühr Y , C. Östman
1985 | 8 (273-280)

Preparation and characterization of subcellular fractions from the intestinal mucosa of the Northern pike (Esox lucius), with special emphasis on enzymes involved in xenobiotic metabolism

Balk, L.; Månér, S.; Meijer, J.; Bergstrand, A.; DePierre, J.W.
1985 | Biochim. Biophys. Acta | 838 (277-289)

The present study was designed to prepare and characterize subcellular fractions from the intestinal mucosa of the Northern pike (Esox lucius), with special emphasis on the preparation of a microsomal fraction suitable for studying xenobiotic metabolism. The purity of the different fractions obtained by differential centrifugation, as well as the recovery of different organelles, was determined using both enzyme markers and morphological examination with the electron microscope. The subcellular distributions of several enzymes involved in drug metabolism (NADPH-cytochrome c reductase, NADH-ferricyanide reductase, epoxide hydrolase activity towards both cis- and trans-stilbene oxide as substrates, and glutathione transferase) were also examined. The subcellular distributions obtained here for drug-metabolizing and marker enzymes closely resembled those reported for rat and pike liver. The microsomal fraction obtained contained about 50% of the total endoplasmic reticulum. This fraction was relatively free of nuclei, mitochondria, Golgi, peroxisomes and cytosol, but relatively heavily contaminated with lysosomes and fragments of the plasma membrane. Within the limitations discussed, the subfractions prepared here are suitable for further characterization of drug-metabolizing systems in the intestinal mucosa of the Northern pike, as well as for other studies with this tissue.

Fractionation and Chemical Analysis of Gasoline Exhaust Particulate Extract in Connection with Biological Testing

Alsberg T., Strandell M., R. Westerholm, Stenberg U.
1985 | 11 (249-258)

Dichloromethane extracts of gasoline vehicle exhaust particulates were fractionated into five fractions according to polarity on a silica gel column. A moderately polar fraction showing high genotoxic activity in the Ames test was further fractionated, using high performance liquid chromatography, on a nitro column, eluted isocratically. Gas chromatography combined with mass spectrometry, flame ionization, and electron capture detection were used for analysis. Also, field desorption mass spectrometry was utilized. Oxygenated derivatives of polycyclic aromatic hydrocarbons, e.g., 9H-fluoren-9-one, alkylfluorenones, and benzofluorenones were the most abundant species in these subfractions. Also, some halogenated aromatic compounds are reported, and quantitative data on nitroarenes are given.

Enrichment of gaseous compounds from diluted gasoline exhausts: A comparison between adsorption and cryogenic condensation

Stenberg U., R. Westerholm, Alsberg T.
1985 | 11 (119-124)

A comparison between an adsorption system and a condensation system for the enrichment of gaseous emissions in gasoline vehicle exhausts is presented. Chemical analyses involve extraction with acetone, fractionation into five fractions, based on different polarity, followed by gas chromatography. The results show that low molecular weight polynuclear aromatic hydrocarbons (PAH) (

MICROCOMPUTER-CONTROLLED 2 SIZE FRACTIONATING AEROSOL SAMPLER FOR OUTDOOR ENVIRONMENTS

HANSSON, HC; NYMAN, S
1985 | Environ. Sci. Technol. | 19 (11) (1110-1115)

The solubility of tri-n-octylamine in water and phosphoric acid solutions

Stenström S, M. Dalene, G. Skarping
1985 | 177 (279-284)

The solubility of tri-n-octylamine (TOA) in water and phosphoric acid is reported. Equilibration is achieved by circulating the solutioin through PVC pieces saturated with the absorbed amine in a glass tube. The dissolved TOA is determined by capillary gas chromatography which can detect amine concentrations down to 10 μg l−1. The solubility of TOA in water is 0.039 mg l−1 and in 5.5 M phosphoric acid 0.82 mg l−1; these values are much lower than those reported in the literature.

CONCEPT AND REALIZATION OF AN AIR-POLLUTION MONITORING STATION IN THE EUROPEAN ARCTIC

HEINTZENBERG, J; HANSSON, HC; OGREN, JA; ODH, SA
1985 | Ambio | 14 (3) (152-157)

Trace analysis of amines and isocyanates using glass capillary gas chromatography and selective detection V. Direct determination of isocyanates using nitrogen-selective and electron-capture detection

G. Skarping, Smith B.E.F, M. Dalene
1985 | 331 (331-338)

The possibilities of direct determination of isocyanates by glass capillary gas chromatography using nitrogen-selective or electron-capture detection was studied in some detail. It was found that only the former method was generally applicable, allowing the quantitative assay of trace isocyanates down to the low picogram range. A comparison with other methods for isocyanate determination, previously developed at these laboratories, was made.

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Stockholm University
106 91 Stockholm

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