Antibiotic-Induced Change of Bacterial Communities Associated with the Copepod Nitocra spinipes

2012 | PLoS ONE | 7 | Report No: e33107

Environmental pressures, such as physical factors, diet and contaminants may affect interactions between microbial symbionts and their multicellular hosts. Despite obvious relevance, effects of antimicrobial contaminants on host-symbiont relations in non-target aquatic organisms are largely unknown. We show that exposure to antibiotics had negative effects on survival and juvenile development of the copepod Nitocra spinipes and caused significant alterations in copepod associated bacterial communities. The significant positive correlations between indices of copepod development and bacterial diversity indicate that disruption of the microflora was likely to be an important factor behind retarded juvenile development in the experimental animals. Moreover, as evidenced by ribotype distribution in the bacterial clone libraries, the exposure to antibiotics caused a shift in dominance from Betaproteobacteria to Cardinium bacteria; the latter have been shown to cause reproductive manipulations in various terrestrial arthropods. Thus, in addition to providing evidence that the antibiotic-induced perturbation of the microbial community associates with reductions in fitness-related traits of the host, this study is the first record of a copepod serving as a host for endosymbiotic Cardinium. Taken together, our results suggest that (1) antimicrobial substances and possibly other stressors can affect micobiome and symbiont mediated interactions in copepods and other hosts, and (2) Cardinium endosymbionts may occur in other copepods and affect reproduction of their hosts.

Ekotoxikologisk testning av avloppsvatten med kräftdjuret Nitocra spinipes med avseende på utveckling, reproduktion och populationstillväxt

Lundström, E.; Brinkmann, M.; Ek, K.; Dahl, U.; Breitholtz, M.
2009 | Rapport Stockholm Vatten

Subchronic (mixture) toxicity testing of 10 selected flame-retardants using the harpacticoid copepod /Nitocra spinipes/.

Breitholtz, M.; Rattfelt, J.; Ek, K.; Karlsson, J.; Andersson, P.
2007

SETAC 17^th Annual Meeting in Europe, Porto, Portugal | September 18, 2019

Ekotoxikologisk testing av avloppsvatten och läkemedelssubstanser med bakterie, alg, kräftdjur och fiskembryon.

Lundström, E.; Hermansson, M.; Linde, M.; Ek, K.; Eklund, B.; Breitholtz, M.
2007 | Rapport Stockholm Vatten

Bedömning av toxicitet av avloppsvatten från Kemwell på tillväxthämning hos rödalgen Ceramium tenuicorne, akut giftighet och larvutveckling hos hoppkräftan Nitocra spinipes samt embryotoxicitet hos spigg- Gasterosteus aculeatus.

Lundström, E.; Stiernström, S.; Ek, K.; Breitholtz, M.
2007 | Report No: Rapport till Kemwell

Metodmanual för tillväxthämningstest med makroalgen Ceramium tenuicorne – en vanlig alg i brackvatten och marina kustzoner. ITM report no. 132.

Eklund, B., Ek, K.
2004 | Report No: 131

Bedömning av toxicitet av avloppsvatten från SCA Packaging Munksund AB på reproduktion och utveckling hos hoppkräftan Nitocra spinipes.

2003 | ITM, SU

Toxicity of zinc run-off wastewaters to the green microalga Raphidocelis subcapitata (Selenastrum capricornutum).

Bengtsson, B-E.; Horg, H.; Ek, J.; Ek, K.; Fröberg, E.; Göthberg, A.; Hjalmarsson, C.; Johansson, C. & Kukulska
1998

Phyto- and zooplankton diversity and algal growth, as indicators of metal stress in mining areas in southern Sweden. Poster.

Hörnström, E.; Ekström, C.; Lithner, G.; Ek, K. & Wahlström
1995

4th International Conference on Aquatic Ecosystem Health: Integrated/Multitrophic Strategies - Microbes to Fish

The development of phyto- and zooplankton in relation to ambient levels of metals (Zn, Cu, Cd, Mn) in mining areas in Southern Sweden. Poster.

Hörnström, E.; Lithner, G.; Ekström, C.; Ek, K.; Fröberg, E.; Matsman, T. & Wahlström
1995

4th Int. Conf. on Aquatic Ecosystem Health

Metodprövning av akuttoxicitetstest med Ceriodaphnia dubia.

Ek, K. & Unger
1993 | ITM, SU

Metodprövning av akuttoxicitetstest med kräftdjur Ceriodaphnia dubia.

Ek, K. & Unger
1992 | SEPA (NV)

Contact information

Visiting addresses:

Geovetenskapens Hus,
Svante Arrhenius väg 8, Stockholm

Arrheniuslaboratoriet, Svante Arrhenius väg 16, Stockholm (Unit for Analytical and Toxicological Chemistry)

Mailing address:
Department of Environmental Science and Analytical Chemistry (ACES)
Stockholm University
106 91 Stockholm

Press enquiries should be directed to:

Stella Papadopoulou
Science Communicator
Phone +46 (0)8 674 70 11
stella.papadopoulou@aces.su.se