Contribution of commonly analyzed polycyclic aromatic hydrocarbons (PAHs) to potential toxicity in early life stages of rainbow trout (Oncorhynchus mykiss)

Sundberg, H.; Ishaq, R.; Tjärnlund, U.; Åkerman, G.; Grunder, G.; Bandh, C.; Broman, D.; Balk, L.
2006 | Can. J. Fish. Aquat. Sci. | 63 (1320-1333)

In a series of bio-effect-directed fractionation experiments, we investigated the potential toxicity of sediment extracts from a contaminated bay. A previous study investigated abnormalities and hepatic ethoxyresorufin O-deethylase (EROD) activities in rainbow trout (Oncorhynchus mykiss) larvae by exposing newly fertilized eggs to the total extract and to fractions separated by degree of aromaticity. A major part of the potential toxicity was isolated in a fraction containing polycyclic aromatic compounds (PACs). In this study, we prepared a synthetic PAC mixture with 17 commonly analyzed polycyclic aromatic hydrocarbons (PAHs) in amounts equimolar to those found in the sediment PAC fraction. The 17 PAHs, which included 11 of the 16 United States Environmental Protection Agency (US EPA) priority PAHs, were unable to account for the toxicopathic effects observed and could explain less than 4% of the total EROD induction. The lack of a clear relationship between toxicopathic effects and EROD induction underlines the need for a battery of biomarkers for estimating environmental risk. These results reveal the limits of our knowledge regarding compounds responsible for potential toxicity in field situations.

Polychlorinated biphenyls in polysulfide sealants – Occurrence and emission from a landfill station

Persson, N.J.; Pettersen, H.; Ishaq, R.; Axelman, J.; Bandh, C.; Broman, D.; Zebühr, Y.; Hammar, T.
2005 | Environ. Pollut. | 138 (18-27)

Dynamics and distribution of hyrphobic organic compounds in the Baltic Sea. In: A systems Analysis of the Changing Baltic Sea. Wulff, F., Rahm, L. & Larsson, P.

Axelman, J.; Näf, C.; Bandh, C.; Ishaq, R.; Pettersen H., Zebühr; Y., Broman
2001 | Springer-Verlag, Germany | Ecol. stud. vol 148 (257-287)
baltic sea , budgets , dynamics and distribution , pah , pcb

Influence of gas-phase/particle speciation in the tropospere on the magnitude of the OH-radical as an environmental sink for PCBs.

Axelman, J.; Bandh, C.; Gustafsson, Ö. & Höglund
2000

SETAC conference

Influence of gas-phase/particle speciation in the troposphere on the magnitude of the OH-radical as an environmental sink for PCBs

Axelman, J.; Bandh, C.; Gustafsson, Ö.; Höglund, S.
2000

3rd SETAC-World Congress

Comparison of accelerated solvent extraction and soxhlet extraction for the determination of PCBs in Baltic Sea sediments.

Bandh, C.; Bjorklund, E.; Mathiasson, L.; Näf, C. & Zebühr
2000 | Environ. Sci. Technol. | 23 (4995-5000)

Patterns and levels of organochlorines (DDTs, PCBs, non-ortho PCBs and PCDD/Fs) in male harbour porpoises (Phocoena phocoena) from the Baltic Sea, the Kattegat-Skagerrak Seas and west coast of Norway.

Berggren, P.; Ishaq, R.; Zebühr, Y.; Näf, C.; Bandh, C.; Broman, D.
1999 | Mar. Pollut. Bull. | 38 (1070-1084)

Organic pollutant characterization and toxicity testing of settling particulate matter by nanoinjection in sea trout (Salmo trutta) eggs

Ishaq, R.; Åkerman, G.; Näf, C.; Balk, L.; Bandh, C.; Broman, D.
1999 | Environ. Toxicol. Chem. | 18 (533-543)

A "nanoinjection" technique was used to expose sea trout (Salmo truttta) eggs to a toluene extract of settling particulate matter (SPM) and fractions thereof from the urban waters of Stockholm, Sweden. Total extract (unfractionated), fractions collected using fractionation on an open Florisil(R) column (F1-F4), high performance liquid chromatography (HPLC) aminopropylsilica/2(1-pyrenyl)ethyldimethylsilulated silica column (A1-A7 and A2/P1-P3), and recombined fractions of Florisil (F-Ret) and HPLC (A-Rec) were injected into fertilized sea trout eggs. Toxicity was evaluated on the basis of deformities at hatching and in larval stages and on the basis of larvae mortality. Chemical analysis, using gas chromatography-mass spectrometry, was performed on all HPLC fractions and indicated that major components of fractions A2/P1 and A3 through A7 were polycyclic aromatic hydrocarbons (PAHs) or their alkylated derivatives. Fraction A2/P2 contained non-ortho-chlorinated biphenyls, polychlorinated dibenzo-p-dioxins and dibenzofurans, and some Cl-substituted PAHs. Deformities at hatching were observed mainly in the larvae exposed to fraction A-Rec. F2 showed the highest effect on larvae mortality, and both F-Rec and A-Rec showed antagonistic mechanisms. The frequency of deformities in larvae was highest in fractions A2/P2, F4, and A-Rec. Fractions A2/P1 and F1, which partly contained similar substances, showed similar effects on deformity at hatching and larvae mortality; however. F1 showed no effect on larvae abnormality compared with A2/P1. This could have been due to antagonistic mechanisms acting in F1.

Inventories and emissions at a PCB contaminated site – Differences in emission rates from aquatic and terrestrial matrices. Oranohalogen Compounds Vol. 36, p.425-428.

Axelman, J.; Broman, D.; Bandh, C.; Burreau, S.; Ishaq, R.; Näf, C.; Pettersen, H. & Persson
1998

18th Symposium on halogenated environmental organic pollutants (Dioxin'98)

Determination of PCBs in Baltic Sea sediments using accelerated solvent extraction (ASE). Organohalogen Compounds Vol. 35, p. 17-19.

Bandh, C.; Björklund, E.; Mathiasson, L.; Näf, C. & Zebühr
1998

18th Symposium on halogenated environmental organic pollutants (Dioxin'98)

Concentrations, spatial variations and biomagnification of organochlorine compounds in sediment and bentic species in the northern part of the Baltic Sea. Organohalogen Compounds Vol 39, p. 419-422.

Strandberg, B.; Bandh, C.; van Bavel, B.; Bergqvist, P-A.; Broman, D.; Ishaq, R.; Näf, C. & Rappe
1998

18th Symposium on halogenated environmental organic pollutants (Dioxin'98)

Concentration, biomagnification and spatial variation of organochlorine compounds in a pelagic food web in the northern part of the Baltic Sea.

Strandberg, B.; Bandh, C.; van Bavel, B.; Bergqvist, P-A.; Broman, D.; Näf, C.; Pettersen, H. & Rappe
1998 | Sci. Total Environ. | 217 (143-154)

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