This project is a joint collaboration with Doc Leopold Ilag and Prof Margareta Törnqvist. Measurement of protein adducts caused by exposure to reactive chemicals, such as electrophiles and many carcinogens, can be an important tool to assess health risks. Identification and quantification of a priori unknown protein adducts require highly sophisticated mass spectrometric methods. We are investigating novel scan techniques, sample preparation methods as well as tags for improved ionization efficiency (Ph D student Javier Zurita). The developed methods may be useful also for identifying the proteins involved in hapten formation at contact allergy.