Per- and polyfluoroalkyl substances (PFASs) constitute a diverse class of anthropogenic chemicals which are ubiquitous in the global environment. Many PFASs are not captured by routine analysis, resulting in an underestimation of exposure to humans and wildlife. The present work utilizes a combination of state of the art analytical techniques, including ultra high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and combustion ion chromatography (CIC) for determining the fraction of organofluorine missed by traditional approaches. Samples with a large quantity of uncharacterized organofluorine are subjected to analysis by high resolution mass spectrometry. This comprehensive approach of mapping the entire organofluorine content in a sample is known simply as ‘fluoronomics’.