The seminar, which is organised in conjunction with ACES, Stockholm University, will cover methodologies of selected areas important and useful for lipid analysis and characterization. The aim is to review each area with examples and applications for industrial and quality control work, as well as for research purposes.
Selected areas:
• Shotgun lipidomics Direct infusion of lipid extracts or samples in the mass spectrometer allow absolute quantification of hundreds of lipid molecular species. This opens interesting possibilities for the analysis of both industrial lipid samples and basic investigation of biological tissues.
• NMR Spectroscopy is an excellent tool to analyse lipid composition in several ways without chemical treatment of the sample. Fatty acid composition based on unsaturation (i.e. quantitative ω-3 determination), quantification of lipid classes (i.e. phospholipids and glycolipids), investiga-tion of oxidation products, and presence of impurities are some examples of applications.
• Supercritical Fluid Extraction (SFE) and Chromatography (SFC) Recent development regarding gas-expanded liquids for chromatography and extraction has turned this field into an interesting option for fats and oils and other fatty components.
• Oxidation Classical methods to investigate the progress of oxidation are in many cases not conclusive or sufficient. Advanced methods such as dynamic headspace analysis can be helpful in order to design the ability for oxidation of lipid products and the strategy for their protection.
• HPLC and GC in lipid analysis These techniques are widely used by lipid analysts. Non-traditional packing materials have made separations of lipids easier and more versatile, which is useful for both quality control and research. Phenyl substituted silica is an example of a packing material useful for all types of lipid separation.
• Chemometrics Modern analytical tools provide a vast amount of data, which increasingly demands for the use of chemometrics or multivariate statistical methods. For the lipid analyst this is highly applicable in quality control work but also for design of methods and experiments.
• Sample preparation In the analysis of lipid samples, it is essential that the procedure does not discriminate between lipid components and is environmentally safe. This has to be achieved by the use of proper extraction and work-up techniques. The choice of solvents for chromatography needs similar consideration to maintain a safe working environment.
Please go to the attached information sheet for more information and a detailed program.
