Found: 7010 publications , showing page 341 of 585
Presentation abstract
Biomarker studies of female perch (Perca fluviatilis) in a chronically polluted gradient through the Stockholm archipelago
Balk, L.; Liewenborg, B.; Linderoth, M.; Sundberg, H.; Noaksson, E.; Hansson, T.; Tjärnlund, U.; Hanson, M.; Schiedek, D.; Åkerman, G.
2003
| International Council for the Exploration of the Sea (ICES)
(ICES CM 2003/M:08)
(1-7)
2003 ICES Annual Science Confrence
| April 18, 2024
| Tallinn, Estonia
Adult female perch (Perca fluviatilis) were investigated at several stations in the Stockholm archipelago in a gradient from Stockholm’s central waterways, through the archipelago to the open Baltic Sea. Various large-scale anthropogenic activities in and around the central part of the city have continuously contaminated especially the inner part of the approximately 80 km long gradient during the past 40 years.
The somatic condition factor, average yearly growth and percentage of sexually maturating females were recorded. Other physiological biomarkers, such as the somatic indices of visceral fat weight, spleen weight, liver weight and gonad weight (GSI) were also included in the study. The biochemical variables investigated were whole blood glucose and haemoglobin, as well as DNA adducts (nuclease P1 version of the 32P-postlabeling methodology), enzymatic activities of CYP1A (ethoxyresorufin O-deethylase), glutathione reductase, glutathione-S-transferase, catalase and glucose-6-phosphate dehydrogenase in the liver and acethylcholinesterase in muscle tissue. Finally, P450arom activity was analyzed in brain tissue.
Our results clearly suggest adverse effects on fish from the stations located within 20-30 km from Stockholm. The GSI and the ratios of sexually mature fish were decreased. The response of many of the biochemical biomarkers indicated an increase in pollution levels as the city is approached. Some of the biomarkers, such as EROD activity and DNA adducts showed, however, surprisingly low responses. Our interpretation of these results is that high chronic exposure has caused physiological and/or genetic resistance to normal biomarker response. In fact, supplementary laboratory studies indicate that this indeed could be the case. In one study, fish from the vicinity of Stockholm did not respond to pure benzo(a)pyrene exposure; in another study, control fish exposed to water/sediment from the area showed a greater response than the feral fish.
Scientific paper
Determination of isoprostanes in Alzheimer urine samples using porous graphitic carbon-HPLC separation and ESI-triple quadrupole MS detection
K. Claeson Bohnstedt, B. Karlberg, L. Wahlund, M. Eriksdotter Jönhagen, H. Basun, S. Schmidt
2003
| J. Chromatogr. B
| 796
(11-19)
F2-isoprostanes (F2-iPs) comprise four classes of isomers produced non-enzymatically by free radical attack on arachidonic acid, a component of the cell membrane. This paper describes a new method for the quantification of F2-isoprostanes in urine samples from thoroughly diagnosed Alzheimer’s disease (AD) patients. The sample pretreatment consisted of liquid extraction of 900 μl urine with diethyl ether, its subsequent evaporation, and finally, reconstitution in 50 μl water. Of this, 20 μl was injected into a HPLC system with a 15 mm x 1 mm porous graphitic carbon column coupled to a triple quadrupole mass spectrometer running in negative electrospray ionization mode. The F2-isoprostanes were separated in 15 min using a linear solvent gradient comprising water, methanol, acetonitrile and ammonium hydroxide at a pH of 9.5. The average recovery obtained was approximately 75%. The limit of detection (3S/N) was calculated for iPF2α-III to be 0.7 pg injected on column, corresponding to 0.1 nM. The average level of iPF2α was 241±163 pg/mg creatinine in the urine samples from AD patients (average±standard deviation). The corresponding control values were 216±101 pg/mg creatinine, i.e. no statistically significant difference was noticed. No correlation pattern specific to Alzheimer’s disease was revealed by principal component analysis of the isoprostane peaks obtained either. The results from this study support earlier findings that levels of peripheral isoprostanes are not increased in patients with Alzheimer’s disease.
PhD/Lic Thesis
Brominated flame retardants and related compounds in Baltic Sea wildlife – Chemical analytical methodology and assessment
Athanasiadou M;
2003
Scientific paper
Effects of four synthetic musks on the life cycle of the harpacticoid copepod Nitocra spinipes.